Effect of celastrol on differentiation and maturation of CD1+4 monocyte-derived dendritic cells
WANG Xuan
LIU Yu-ying
PENG Mei-yu
XUE Zhen-yi
Abstract：Objective To Make the differentiation and activation models of dendritic cells ( DCs ) and to investigate the effect of celastrol on the differentiation and maturation of human DCs in vitro .Methods Fresh human buffy coat was obtained , and peripheral blood mononuclear cells were isolated .Purified CDl4+monocytes were analyzed by FACSCalibur to confirm the purity of CD1+4 cells.The purified CD1+4 cells were cultured in complete 1640 medium supplemented with GM-CSF and IL-4 for 5 days and 7 days.For DC maturation, cells were stimulated with 5?g/mL lipopolysaccharide (LPS) at day 5.The control group was not added celastrol , but the celastrol group was added 10 and 20 nM celastrol, respectively. Then immature DCs ( iDC ) and mature DCs ( mDC ) were collected to detect the mean fluorescent intensity ( MFI ) of CD80 , CD83 , CD86 , HLA-DR and the absorb capacity of FITC-dextran by flow cytometry .Results Compared with the control group, the MFI of CD80 , CD83 , CD86 and HLA-DR of iDC and mDC in the celastrol group was lower , and the treat-ment of 20 nM was lower than the treatment of 10 nM, all P<0.05.The MFI of FITC-dextran in the negative control group (10.1 ±2.1) was the lowest, and was the highest in control group (243.7 ±31.3);in addition, the MFI of FITC-dextran in the 20 nM celastrol group (72.4 ±10.2) was lower than that of the 10 nM celastrol group (186.3 ±22.6), all P<0.05.Conclusions Celastrol inhibits CD1+4 monocytes differentiating into DCs , further inhibits phenotypic maturation of LPS-induced DCs , and suppresses the antigen-presenting function of iDC .
Keywords：dendritic cellsdifferentiationmaturationendocytosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 20-22 )