Association between hypoxia-inducible factor-2αand hepatitis B virus x protein expression in hepatocellular carcinoma tissues and cells
CHENG Quan-sheng
AN Xiang
LIU Ping
LIU Xin-quan
YAO Hong-yi
WU Chao
Abstract：Objective Objective To investigate the relationship between hypoxia-inducible factor-2α( HIF-2α) and hepatitis B virus X protein (HBx) in hepatocellular carcinoma (HCC) tissues and cells.Methods The HCC Bel-7404 cell line was cultured and then was divided into 6 groups:groups A, B and C were transfected by 1.2, 0.6, and 0.3 μg pEGFP-HBx;the no-load transfection group was transfected by 1.2 μg pEGFP; the control group was only added lipido-some;and the non-transfection group was not added any lipidosome and plasmids .Real-time PCR and Western blotting were used to investigate the mRNA and protein expression of HIF-1αand HIF-2α.Immunohistochemistry was used to de-tect the expression of HIF-2αand HBx in 33 HCC tissues , and their possible relationships were analyzed by using SPSS 15.0 statistical software.Results The mRNA and protein expression of HIF-1αand HIF-2αin Bel-7404 cells:group A>group B >group C >no-load transfection group >control group >non-transfection group (all P<0.05).The ex-pression of HIF-1αand HIF-2αwas increased in Bel-7404 cells at both mRNA and protein levels in a dose-dependent man-ner after being transfected by HBx plasmid (all P<0.05).HIF-2αwas highly expressed in HCC tissues , but was not cor-related with the expression of HBx (P>0.05).Conclusion HBx may up-regulate the expression of HIF-2αand HIF-1αin HCC cells, but it is not correlated with HIF-2αexpression in HCC tissues .
Keywords：hypoxia-inducible factorhepatitis B virus X proteinhepatocellular carcinoma
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )