Contrast-enhanced ultrasound quantitative analysis technique in evaluating renal medulla perfusion of rats with unilateral ureteral obstruction

YANG Qin; YAO Xi-cun; SUN Hao-ran

doi:10.3969/j.issn.1002-266X.2014.16.003

Abstract：Objective To evaluate the renal medulla blood perfusion characteristics in rats with unilateral ureteral ob -struction (UUO) by using contrast-enhanced ultrasound (CEUS) and quantitative analysis technique .Methods Nineteen UUO rat models were established , and the bilateral renal medullary perfusion before and after the UUO operation was detec -ted by using CEUS and quantitative analysis technique , meanwhile , pathology examination was performed .Results After modeling , the blood perfusion velocity of the obstructed kidney medulla was slower than before modeling , the contrast agent washout time was prolonged , but the enhancement degree changed little;while, the blood perfusion velocity , intensity and contrast agent washout time changed little before and after the modeling of the normal renal medulla .Quantitative analysis showed that the preoperative and postoperative blood perfusion time to peak ( TTP) in the obstructed kidney medulla were (25.59 ±3.92) and (28.48 ±4.67)s, area under curve (AUC) were (905.46 ±271.90) and (1 010.54 ±309.11) dB · s, and slope rate of ascending curve (A) were (1.04 ±0.59) and (0.87 ±0.41)dB/s, respectively (all P<0.05). No significantly differences were found in TTP, AUC, A and PI of the normal renal medulla (P>0.05).The obstructed renal proximal tubule and distal convoluted tubules showed varying degrees of expansion , broadening renal interstitial ede-ma, and infiltration of mononuclear macrophage and lymphocyte , occasionally tubular atrophy .Conclusions The obstruc-ted renal medulla blood perfusion velocity of UUO rats decreases and intravascular blood volume increases .CEUS and quantitative analysis technique may effectively detect the changes of renal medulla perfusion in UUO rats .