Influence and significance of mild moxibustion on collagen type I expression in ovariectomized rat femur model
WANG Jun-yan
YANG Xiao-hui
LIU An-guo
DONG Li-li
MA Chong-bing
ZHU Tian-tian
YAN Xing-ke
Abstract：Objective To observe the expression changes of collagen typeⅠin ovariectomized rat femur model and the intervention effects of mild moxibustion and estradiol benzoate injection ( BE2 ) , and to investigate possible mechanism of tonifying deficiency and dissolving blood stasis in treating postmenopausal osteoporosis (PMO).Methods Of 50 female Wistar rats at 8-month old, 10 were randomly allocated to the sham group and other 40 underwent the ovariectomized opera-tion.After 90 days, the ovariectomized female Wistar rats were randomly divided into 4 group, 10 in each group:ovx group ( model group ) , estrogen group , moxibustion group and moxibustion estrogen group .The rats in the sham group and ovx group did not received any treatment .The rats in the estrogen group received the treatment of muscle injection of estradiol benzoate in the leg .The rats in moxibustion group received the treatment of mild moxibustion according to the method of "tonifying deficiency and dissolving blood stasis"to select points .The rats in moxibustion estrogen group received the treat-ment of the mild moxibustion and muscle injection of estradiol benzoate .The rats were killed at the end of course to observe bone collagen typeⅠand bone collagen typeⅠgene mRNA expression by using immunohistochemistry and in situ hybridiza-tion.Results The expression of collagen type Ⅰand collagen typeⅠmRNA in the model group was significantly lower than that of the sham group and other treatment groups;during the treatment groups , the positive reaction area in the moxi-bustion estrogen group was significantly larger than that of the moxibustion group (P<0.05 or P<0.01), and the other in-dexes had no difference .Conclusion The mild moxibustion can improve the expression level of collagen typeⅠand colla-gen typeⅠmRNA of the ovariectomized rats , and this may be one of the mechanisms of mild moxibustion by tonifying defi-ciency and dissolving blood stasis in treatment of PMO .
Keywords：postmenopausal osteoporosismild moxibustiontonifying deficiency and dissolving blood stasiscollagen typeⅠcollagen typeⅠmRNA
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 24-27 )