Effect and mechanism of benzyl isothiocyanate on cell cycle of human glioma cell line U-87 MG
LI Wen-ming
WU Qi
ZHU Yu
Abstract：Objective To investigate the effect and mechanism of benzyl isothiocyanate ( BITC) on arresting cell cy-cle of human glioma cell line U-87 MG.Methods U-87 MG cells in the logarithmic phase were treated with 2 and 5μmol/L BITC.The cell cycle distribution was measured by flow cytometry , the expression of cell cycle related proteins Cy-clin B1, Cdc2, p21 and Cdc25C, and the phosphorylation of Akt was measured by Western blotting and RT-PCR, the tran-scriptional activity of NF-κB, HIF and eIF4E was determined by reporter gene system .Results After BITC treatment for 24 h, the proportion of cells in the G2/M phase of cell cycle was increased, and the protein expression of Cyclin B1, Cdc2, Cdc25C and p-Akt was down-regulated, but in contrast p21 was elevated; the mRNA expression of Cyclin B1, Cdc2, Cdc25C and p21 was down-regulated, and the transcriptional activities of NF-κB, HIF and eIF4E were significantly de-creased (all P<0.05).Conclusions BITC is able to arrest cell cycle of human glioma cell line U-87 MG.The mecha-nism may be related to the inhibition of Akt/NF-κB pathway and thus regulating cell cycle related gene expression , which provides a new idea for the target treatment of glioma cell cycle .
Keywords：benzyl isothiocyanategliomacell cycle
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )