Influence of modified silicone membrane on proliferation and differentiation of neural stem cells
YANG Xiao-qing
CHEN Xu-yi
CHENG Shi-xiang
LIU Ying-fu
LIU Chun-rong
LI Rui-xin
LI Na
LI Jian-guo
Abstract：Objective To explore the influence of silicone membrane on the neural stem cells'( NSCs) proliferation and differentiation, and to provide an experimental basis for the research of NSCs'carrier.Methods We selected 16 d pregnant rats cerebral cortex as the source of the cells , and established embryonic neural stem cells (NSCs) culture system in vitro. Then NSCs were randomly divided into the observation group and the control group , the observation group was inoculated on modified silicone membrane , and the control group was inoculated on the ordinary culture dish .The cell growth morphology of NSCs was observed by using light microscope and electron microscope .The NSCs and differentiated cells were identified by immunofluorescent staining , and MTT assay was used to detect the proliferation .Results After culture for 2 d, neural spheres were formed and adhered well , neuritis was detected from the assay , and nestin-positive was detected in both groups . After 7 d, NSE-positive rates of the experimental group and control group were 25.14%±1.98%and 24.67%±2.05%;GFAP-positive 59.48%±2.07%and 60.87%±1.08%(all P>0.05).The growth curve of the two groups showed no sig-nificant difference (P>0.05).Conclusions The modified silicone membrane has no significant effect on the NSCs'growth, proliferation and induced differentiation but promotes the growth of neurite between cells , which can be used as a culture car-rier to study the effects of mechanical factors on NSCs'proliferation and differentiation .
Keywords：neural stem cellsinduced differentiationsilicone membrane
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 8-10 )