Effect of Chk1 gene silencing on radiosensitivity of ovarian cancer cells
SUN Dong-yan
WANG Yan
YAO Dong-mei
ZOU Qian
LANG Yan
DUAN Jie
FANG Lin
Abstract：Objective To investigate the effect of Chk 1 gene silencing on the proliferation and radiosensitivity of hu-man ovarian cell line A2780.Methods Vectors containing short hairpin RNA (shRNA) targeting Chk1 gene were con-structed and transfected into human ovarian carcinoma cell line A 2780 .At the same time , transfecting the empty vector and untransfected group were selected as controls .The RT-PCR and Western blotting were used to compare the differences of Chk1 expression before and after trasfection .The cell growth curve was drawn by methyl thiazolyl tetrazolium ( MTT);and the radiosensitivity of ovarian carcinoma was evaluated by Clone formation array .Results Compared with the control group, constructing shRNA expression vector inhibited the Chk 1 mRNA transcription and protein expression of human ovari-an carcinoma cell line A2780;after Chk1 gene silencing , the cell growth rate was obviously slower than that of the control group;the radiosensitivity of A2780 cells was enhanced by decreasing the G 2/M phase blocking.Conclusion The shR-NA targeting at Chk1 gene can efficiently inhibit the Chk 1 expression in A2780 cells, which can enhance the apoptosis and obviously increase the radiosensitivity in human ovarian cell lines .
Keywords：ovarian neoplasmsRNA interferenceradiosensitivityapoptosisChk1
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 6-9 )