Inhibitory effect of N-isoquinoline-3-carbonyl-L-Glycine-benzylester on human esophageal carcinoma cells in vitro and in vivo
YANG Guo-dong
ZHANG Xiao-ying
FENG Zhi-song
REN Quan
JIANG Yi-min
Abstract：Objective To investigate antitumor ability of N-isoquinoline-3-carbonyl-L-Glycine-benzylester ( IQ-G-OB-zl)on the human esophageal carcinoma cells in vitro and in vivo .Methods ①In vitro test:there were four groups in vitro test and esophageal carcinoma cells were treated with 5-FU, (L-Gly-OBzl), IQ and IQ-G-OBzl, respectively;MTT assays were performed to assess the IC 50;Intracellular reactive oxygen species ( ROS) were detected by flow cytometry .②In vivo test:the model of esophageal carcinoma in 115 nude mice was established;there were five groups in vivo test ( NS, IQ-G-OBzl, L-G-OBzl, IQ and IQ-G-OBzl) and were treated with each compound respectively .then weight of tumor was meas-ured, the inhibition rate of tumor was calculated .Results In vitro test, the IC50 of IQ-G-OBzl group was higher than that of 5-FU group, but less than that of L-Gly-OBzl and IQ groups(P<0.05).In vivo test, the inhibition rate of IQ-G-OBzl group was higher than that of L-Gly-OBzl and IQ groups (all P<0.05);the ROS level of IQ-G-OBzl group was significant-ly higher than that of L-Gly-OBzl, IQ and NS groups(P<0.05).Conclusion IQ-G-OBzl has anti-esophageal carcinoma ability in vitro and vivo , and it has no obvious side effects , whose mechanism may be related to the increasing level of ROS.
Keywords：N-isoquinoline-3-carbonyl-L-Glycine-benzylesteresophageal carcinomareactive oxygen species
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-20 )