Inhibitory effect of Notch1 siRNA on proliferation and tumor growth of malignant melanoma cells in vivo and in vitro
QI Yan-xia
KANG Shi-jun
LIU Xin-hui
LUO Rong-cheng
Abstract：Objective To observe the inhibit effeet of siRNA on Notch 1 in mouse malignant melanoma cells in vivo and vitro.Method We used siRNA targeting Notch1 gene to transfect melanoma cell line B16F1and induced RNA inter-ference.RT-PCR, Western blotting, MTT method and cell cloning assay in vitro were used to detect the expression of Notch1 gene and protein expression , cell proliferation and cell clone formation changes .Then we transfected or inoculated the control cells subcutaneously into syngeneic C 57BL/6 mice, and in the intratumoral process , the corresponding siRNA was injected .The tumor growth was recorded and the growth curve was drawn .After that , we executed the animal to get tumor and compared the tumor volume .Meanwhile, HE and immunohistochemical staining were used to observe the patho-logical changes of tumor tissue and the expression of Notch 1 protein.Results The cell proliferation , clone formation rate and tumor formation in mice were inhibited after Notch1 siRNA transfecting B16F1 cells (P<0.01), and immunohisto-chemistry showed that Notch1 protein positive cell percentage of the siNotch 1 transfected group was lower than that of con-trol group (all P<0.01).Conclusions Targeting Notch1 gene siRNA can inhibit the proliferation and growth of mouse melanoma cells by decreasing the expression of Notch 1, which can be used as a new method for the treatment of malignant melanoma.
Keywords：malignant melanomamicecell proliferationtargeting Notch1 gene small interfering RNA
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 14-16,20 )