Effect of Dahuang Mudan decoction-contained serum on the expression of TLR4 and MyD88 in lipopolysaccharide-stimulated pulmonary macrophages of rats
SUN Yan-ni
CHENG Jie-jing
YANG Xiao-yan
YANG Jie
WANG Li-min
LIU Jun
Abstract：Objective To investigate the therapeutic mechanism of Dahuang Mudan decoction-contained serum ( here-inafter referred to as the drug contained serum ) in the treatment of sepsis .Methods Mouse pulmonary macrophage cell line RAW264 .7 was cultured in vitro , and then the cells were randomly divided into the blank group , model group , high-, moderate-and low-concentration drug-contained serum groups , each group of 3 holes.Except the blank group , other four groups were stimulated with bacterial lipopolysaccharide ( LPS) 5 μg/mL, then were treated with different concentrations of drug-contained serum for 24 h.After the intervention , the cells were cultured in 5%CO2 at 37℃for 24 h.Then the mR-NA expression of Toll-like receptor 4( TLR4) and myeloid differentiation factor 88( MyD88) was measured by semi-quanti-tative reverse transcription-polymerase chain reaction ( RT-PCR ) , and their protein expression was analyzed by Western blotting .Results The protein and mRNA expression of TLR 4 and MyD88 in the model group was significantly higher than that of the blank group (P<0.01), but the protein and mRNA expression of TLR4 and MyD88 in the high-and moderate-concentration drug-contained serum groups was significantly lower than that of the model group (P<0.01), and no signifi-cant differences was found between the high-and moderate-concentration drug-contained serum groups .Conclusion The therapeutic mechanism of Dahuang Mudan decoction-contained serum in the treatment of sepsis may be related to the inhibi-tion of TLR4 and down-regulation of MyD88 expression.
Keywords：Dahuang Mudan decoctiondrug-contained serumpulmonary macrophageToll-like receptor 4myeloid differentiation factor 88
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 10-13 )