Expression of nestin mRNA in umbilical cord mesenchymal stem cells
HOU Ji-feng
YAO Xing-yu
ZHANG Guo-hua
ZHAO Peng-wei
YANG Li-min
Abstract：Objective To observe the expression of nestin nestin mRNA in umbilical cord mesenchymal stem cells (MSCs) from the perspective of gene .Methods The fresh umbilical cord tissues underwent MSCc separation and then were cultured and purified .Cells from the umbilical cord were counted by enzymatic digestion , and the activity was detec-ted by flow cytometry .The primary, first-generation and second-generation cellular mRNA were extracted to detect the ex-pression of nestin mRNA by real-time PCR.Results The cells isolated from umbilical cord grew in good condition , in which a large part of the cells grew in fusiform , cells after passage had the similar morphology with primary cells and grew faster.There were individual cells which were inneural-like growth.Nestin mRNA expression was found in umbilical cord MSCs, and the expression level after the passage was significantly decreased , meanwhile, the nestin protein changes were consistent with that of differentiation from the neural precursor cells into neural cells .Conclusion The nestin mRNA ex-pression was found in the MSCs tissue genes .Even the expression level of nestin mRNA decreases after passage , it still can be used as an alternative seed cells for transplantation .
Keywords：mesenchymal stem cellsumbilical cordnestinreal-time fluorescence quantitative polymerase chain reaction
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )