Establishment of a human prostatic carcinoma cell line stably overexpressing Kallikrein 7
LIN Zhi-di
MO Lin-jian
ZHANG Cheng-dong
XUAN Qiang
ZHANG Yue-ning
MO Zeng-nan
TENG Ruo-bing
YANG Xiao-li
Abstract：Objective To establish a human prostatic carcinoma cell line stably overexpressing Kallikrein 7 (KLK7) and provide foundation for prostate cancer pathogenesis .Methods KLK7 cDNA was amplified by PCR and cloned into the eukaryotic expression vector pcDNA3.1.Then it was confirmed by restriction endonuclease and DNA sequencing test , and the correct vectors were transfected into prostate cell line DU 145 with lipofectamine .The survival cell clones treated by G418 were amplified.The KLK7 expression of cell line DU145 was detected by Western blotting in each cell clone .Re-sults After restriction endonuclease analysis and DNA sequencing , the pcDNA3.1-KLK7 eukaryotic expression vector was successfully constructed .The stably transfected DU145 cell line highly expressing KLK7 was successfully established after being transfected .The results of Western blotting showed the expression of KLK 7 protein was significantly higher in stable transfectants with the KLK7 vector than that in the cells with empty vector (P<0.01).Conclusion The construc-tion of the eukaryotic expression vector pcDNA 3.1-KLK7 and the establishment of stable prostatic cell line overexpressing KLK7 provide basis for further studies on the function KLK 7 in the prostate pathogenesis .
Keywords：recombinant human kallikrein 7prostate carcinomastable cell lineeukaryotic expression vector
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )