Effect of glucose fluctuation on CD11b and TNF-αproduced by monocytes
SONG Hua-jing
KOU Wei
JIANG Xue-ling
LI Juan
QI Wen-bo
Abstract：Objective To investigate the effectss of fluctuation of glucose concentrations on CD 11b and tumor necrosis factor ( TNF)-αproduced by Mmonocytes , and discuss the role of the blood glucose in the pathogenesis of diabetic mac-roangiopathy .Methods The THP-1 cell strain was randomly divided into the control group , high glucose group , fluctua-ting glucose concentration group and osmotic pressure control group .The expression of CD11b on the surface of monocytes was detected by flow cytometry .The concentrations of TNF-αwere detected by ELISA and then were compared in each group.Results The concentrations of TNF-αand CD11b in the high glucose group were significantly higher than those in the control group, the levels of TNF-αand CD11b of the fluctuating glucose group were higher than those of the high glu-cose group, and the concentrations of TNF-αand CD11b of the osmotic pressure control group were between those of the high glucose group and the fluctuating glucose group .The differences between groups was statistically significant ( all P<0.05).Conclusion The fluctuation of glucose can promote monocytes secreting more CD 11b and TNF-α, which can pro-duce a greater cellular toxicity than simple high glucose does .
Keywords：monocytesglucoseCD11btumor necrosis factor-α
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 28-30 )