DMRIE-C liposome mediated CLEC2 B transfects into Jurkat cells and the influence factors of transfection rate
LIU Jun-ru
ZHANG Jun-ling
XU Shi-fu
CHENG Lin
Abstract：Objective To observe the process of transfecting CLEC 2B siRNA mediated by DMRIE-C liposome into the Jurkat cells and to analyze the factors of influencing transfection rate .Methods We transfected CLEC2B siRNA into the Jurkat cells by DMRIE-C.Trypan blue staining was used to observe the cell activity .siRNA transfection efficiency was de-tected by flow cytometry , and the CLEC2B gene silencing efficiency was detected by RT-PCR.Results The cell damage degree in the transfection process was influenced by the cell members /transfection system lipo concentration ratio , 3μl lipo in the transfection system (600 μl total liquid, 200 μl 2 ×106/ml lymphocytes) would damage cells, and go against the following experiment.The transfection efficiency could increase to 30.70%when the siRNA:DMRIE-C ratio was adjusted to 3 μl∶2 μl, and then, the CLEC2B gene expression inhibition ratio was about 35 ±1.9%(t=15.9, P<0.05).Con-clusion DMRIE-C lipo cloud successfully transfer aim siRNA to Jurkat cells , and meanwhile, lipo/lymphocyte ratio, ser-um interference and operation skills may influence the transfection efficiency .
Keywords：liposomesDMRIE-Csmall interfering RNAJurkat cells
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 20-22 )