Dynamic proteomics of plasma in patients with diabetic nephropathy
WANG Yan-hui
FU Ming-jie
HUANG Yu-ning
LI-Jing
Abstract：Objective To investigate the differential expression of plasma protein in patients with diabetic nephropathy ( DN) and to provide a basis for finding diabetic nephropathy sensitive plasma protein markers .Methods Four DN pa-tients and 4 healthy people were included in this study .The plasma of the included patients were extracted and tested for differential expression by using 2-DDIGE.Higher or lower than 1.5 times was considered as the cutoff filter to screen the differentially expressed protein spots which would be analyzed by mass spectrometry .Results We successfully constructed the two-dimensional gel electrophoresis profiles of plasma protein differential expression of patients with DN , and 6 different expression plasma proteins were identified .By mass spectrometry and UniProtKB/Swiss-Prot, we identified 6 proteins, in-cluding complement C3, C4, gelsolin precursor, fibrinogenγ, apolipoprotein E and cytoskeletal protein 16.Conclusion The differential expression plasma protein between DN patients and healthy people can by identified by bidirectional fluores -cence difference gel electrophoresis , which provides the basis for further screening DN plasma protein markers.
Keywords：diabetic nephropathyplasma proteinproteome
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )