Effects of chrysotile and its substitute on p16 and p53 expression of V79 hamster cells
WANG Hong-zhou
DENG Li-juan
WANG Li-min
DONG Fa-qin
Abstract：Objective To investigated the effects and possible carcinogenic mechanism of chrysotile and its substitute on p16 and p53 expression of V79 hamster cells.Methods Chinese hamster lung cells (V79 cells) were exposed to the suspension of chrysotile and its substitute [ ( Xinkang Sichuan ( A ) , chrysotile from Southern Shanxi ( B ) , glass fiber (C), ceramic fiber (D), wollastonite (E) and rockwool (F) groups and control group (G)] with different powder con-tent (1-10 mg/L).The p16 and p53 expression in V79 cells was tested by immunohistochemistry method , then p16 and p53 statistics were compared .Results Compared with the control group , the antioncogene expression of p 16 and p53 was down-regulated in V79 cells of the six experiment groups (P<0.05).With the increase of exposure concentrations , the expression of p16 and p53 were decreased and OD value of p16 and p53 were decreased (all P<0.05).Conclusions Chrysotile and its substitute show different levels of carcinogenicity .The carcinogenic mechanism of chrysotile and its sub-stitute may be related to down-regulation of the tumor gene expression of p 16 and p53 .
Keywords：chrysotilesubstitutetumor genegenes expression regulationneoplasticgenesp16p53
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )