Expressing and ability protecting primary dopaminergic nerous of human GDNFcDNA in engineered SH-SY5Y cells
Abstract：To construct a kind of engineered cells that can secrete human glial cell line-derived neurotrophic factor（GDNF）and study its possible effects on gene therapy of Parkinson’s disease.Cloned the GDNFcDNA with Kozak sequence by RT-PCR，and then transfected it into SH-SY5Y cell line，co-cultured the engineered cells with primary mesencephalic cells.Results showed that the engineered cells showed obvious protecive effects such as anti-degeneration and anti-MPP＋lesion.GDNF produced by the engineered cells was of full biological function.It suggests that the engineered SH-SY5Y cells can secrete human GDNF and could play an important role in gene therapy of Parkinson’s disease.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 1-2 )