The effect of insulin therapy on the ultrastructure of pyramidal neuron in temporal cortex of the streptozotocinn induced diabetic rats

doi:10.3969/j.issn.1002-266X.2001.06.001

Abstract：The effect of insulin therapy on the ultrastructure of pyramidalneuron in temporal cortex of the streptozotocin induced diabetic rats was observed under light and electron microscope for 3 months.The histology of pyramidal neurons in temporal cortex was not obviously different among three groups：controls，untreated diabetics and diabetics treated with insulin.The ultrastructral changes was found in untreated diabetics.The nuclear membrane inveginated and the nuclei lost their normal shape.The nuclear inclusions were distributed unevently.Some heterochromatin gathered near the nuclear membrane.The number of the main organelles in the cytoplasm decreased.The rough endoplasmic reticula（rER）became thicker and shorter.The cavities of rER were irregular；Parts of them were dilated.The loss of some ribosomal particles from the rER can also be seen.With dilated cavities and disappeared porlarites，the shape of Golgi′s bodies became irregular.The cristae of mitochordria were less and some of them became indistinct or disappeared；Some double membrane were not distinguishable clearly.However，the ultrastructure in diabetic rat treated with insulin were normal.It is suggested that the ultrastructral damage in temporal cortex observed in the study was induced by diabetes.The ultrastructral change provide the morphological evidense for the cognitive dysfunction observed in diabetes.The damages can be prevented and treated with observed in diabetes.The damages can be prevented and treated with insulin.