Transcriptional Regulation Mechanism of Flavonoid Accumulation in Different Germplasms of Scutellaria baicalensis Based on Metabolomics and Transcriptomics
Wang Tingshuo
Guo Fengdan
Zhao Yiwu
Zhou Xuan
Xin Changjian
Lin Huibin
Xu Lili
Guan Renwei
Abstract：Scutellaria baicalensis,a vital medicinal plant,contains flavonoids in roots with diverse phar-macological activities.To investigate the differential accumulation mechanism of flavonoids among different germplasms,the white-flowered,pink-flowered,blue-purple-flowered and purple-flowered S.baicalensis germ-plasms were selected for the integrated metabolomics and transcriptomics analyses to identify differential metab-olites and genes.Through metabolomic analysis,1 823 metabolites were detected including 576 flavonoids,a-mong which,35 significantly different flavonoid metabolites were identified,such as naringin chalcone and ru-tin in purple-flowered S.baicalensis,and kaempferol in white-flowered S.baicalensis.Through transcriptomic sequencing,5 331 differentially expressed genes(DEGs)were identified,including 33 genes involved in fla-vonoid biosynthesis pathways like CHS,ANS,FLS and AOMT.Additionally,MYB,ERF and bZIP transcrip-tion factor families were predicted to regulate flavonoid synthesis-related gene expression.Eight DEGs related to flavonoid biosynthesis were randomly selected for the qRT-PCR validation,and the results corroborated the transcriptomic data.This study elucidated the molecular mechanisms of flavonoid accumulation differences a-mong different flower color S.baicalensis germplasms,which could provide theoretical foundations for germ-plasm improvement and secondary metabolite regulation.
Keywords：Scutellaria baicalensisFlavonoidsMetabolomicsTranscriptomicsDifferential accumula-tionRegulatory mechanism
Publication Date：2026-02-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：12( 1-12 )