Dynamic Changes of Cold Tolerance and Main Biochemical Substances of Eumerus sp.before and after Overwintering

Wang Guiping; Liu Haigang; Wang Bencai; Song Zhongxing; Liu Dahui; Zhang Kai; Yu Yi

doi:10.14083/j.issn.1001-4942.2026.01.017

Abstract：The Eumerus sp.is a major pest that attacks the tubers of Gastrodia elata Blume.To clarify the cold resistance of Eumerus sp.,this study determined and analyzed the supercooling point and freezing point,as well as the contents of major biochemical substances such as water,trehalose,glycogen,fat,and protein in five life stages of Eumerus sp.including mature larva,pre-pupa,floating pupa,settling pupa and a-dult before and after overwintering.The results showed that the supercooling point in the overwintering under-ground mature larva and pre-pupa was significantly higher than that in the surface-floating pupa,surface-set-tling pupa and adult in spring,and the freezing point in the mature larva and adult was significantly higher than that in pre-pupa,floating pupa and settling pupa.The water content in mature larva and adult was signifi-cantly lower than that in pre-pupa,floating pupa and settling pupa.The trehalose content in mature larva and adult was significantly higher than that in pre-pupa,floating pupa and settling pupa.After overwintering,the glycogen in each stage showed a trend of gradual decline.The fat and protein contents in mature larva,pre-pu-pa and floating pupa were significantly lower than those in settling pupa and adult.In summary,the cold re-sistance of different stages of Eumerus sp.was closely related to the location before overwintering.The cold re-sistance of underground forms of Eumerus sp.was significantly lower than that of surface insect form,and the physiological and biochemical metabolism within its body underwent corresponding changes,in order to adapt to the unfavorable ecological conditions of winter and spring and ensure normal development.