Effects of Straw Returning with Low-Temperature Tolerant Compound Microbial Agent HT20 on Soil Extracellular Enzyme Activity and Bacterial Community Structure
Sun Jianping
Li Hao
Zhao Zijing
Liu Yahui
Dai Xianglin
Abstract：To elucidate the impacts of straw returning combined with the low-temperature tolerant com-pound microbial agent HT20 on soil biological properties in the Jidong rice region,a field experiment was con-ducted with three treatments including no straw returning(CK),straw returning without HT20(J0),and straw returning with HT20(JH),and their effects on soil extracellular enzymes,cellulose-degrading genes,and bacterial community structure and function were analyzed by high-throughput sequencing together with flu-orimetric determination and quantitative real-time PCR.The results demonstrated that,compared with JH treatment,CK and J0 treatments significantly inhibited the activities of soil extracellular enzymes as well as the abundances of two key cellulose-degrading genes,cbhI and GH48,at several growth stages(P<0.05).Specif-ically,the activity of α-glucosidase decreased by 19.52%and 26.12%at transplanting stage,and 19.79%and 9.02%at maturity stage,respectively.The activity of β-glucosidase reduced by 23.58%and 18.74%respectively at tillering stage.The activity of β-xylosidase reduced by 39.70%and 2.96%at transplanting stage,59.89%and 25.09%at tillering stage,and 20.53%and 25.33%at maturity stage,respectively.Meanwhile,the abundance of cbhI gene was diminished by 83.33%and 44.93%at transplanting stage,53.28%and 15.86%at booting stage,66.03%and 25.80%at milk-ripening stage,and 72.53%and 41.93%at maturity stage,respectively.The abundance of GH48 gene decreased by 71.83%and 69.51%at transplanting stage,65.36%and 56.28%at tillering stage,and 68.43%and 42.71%at milk-ripening stage,respectively.Moreover,JH treatment al-tered the bacterial community structure and functional traits,which was dominated by Actinobacteria,Pro-teobacteria,and Firmicutes.LEfSe analysis identified 4,2 and 8 indicator taxa in JH,J0 and CK,respective-ly.BugBase phenotype prediction revealed that the JH treatment enriched traits related to Gram-positive bacte-ria,biofilm formation,pathogenicity reduction,and anaerobiosis.These findings provided molecular-level in-sights into the accelerated straw decomposition facilitated by HT20 in paddy soils,and could offer a theoretical basis for developing novel low-temperature straw-decomposing agents.
Keywords：Low-temperature tolerant compound microbial agent HT20Straw returningSoil extracellu-lar enzymesCellulolytic genesMicrobial function
Publication Date：2026-01-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：11( 93-103 )