Isolation,Identification and Genetic Evolution Analysis of Porcine Reproductive and Respiratory Syndrome Gene Type 2 Lineage 8 Strain
Han Yu'er
Yu Jiang
Zhu Chenguang
Li Jianda
Zhang Yuyu
Zeng Hao
Xu Yulin
Liu Fei
Chen Zhi
Sun Wenbo
Liu Jianchai
Wu Jiaqiang
Abstract：In order to understand the current epidemic status and genetic evolution of porcine reproduc-tive and respiratory syndrome virus(PRRSV)in Shandong Province,the pathogens were isolated and identi-fied through inoculating the PRRSV positive samples into Marc-145 cells after filtration.Indirect immunofluo-rescence was used to identify the cell samples that had passed to the fifth generation and had cytopathic effect.The key genes of PRRSV strains were amplified by RT-PCR,including ORF5,ORF7 and NSP2,and the am-plified products were sequenced and compared with the published PRRSV sequences in NCBI.The results showed that a strain of PRRSV named ZB20231123B was successfully isolated from the samples.When inocu-lated into Marc-145 cells,the strain could induce typical cytopathic effect.The nucleotide homology analysis showed that the isolate ORF5 sequence shared 83.6%to 99.7%nucleotide homology with the known PRRSV strains,and the ORF7 sequence shared 89.8%to 100%nucleotide homology with the known American-type PRRSV strains.The nucleotide homology of NSP2 sequence with known American-type PRRSV strains was 76.5%to 99.8%.Phylogenetic analysis further confirmed that the isolate ZB20231123B was on the same evo-lutionary branch with PRRSV lineage 8 strain in China and abroad.In conclusion,ZB20231123B was a typical PRRSV gene type 2 lineage 8 strain.
Keywords：Porcine reproductive and respiratory syndromeLineage 8IsolationIdentificationGenetic evolution
Publication Date：2025-12-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 131-138 )