Structural Changes of Soil Ascomycetes Community during Halophytic Vegetation Succession in the Yellow River Delta

Guo Jian; Wang Xiaofeng; Guo Dufa

doi:10.14083/j.issn.1001-4942.2025.11.013

Abstract：In order to understand the response of ascomycetes community structure to halophytic vegeta-tion succession and explore the effects of environment factors on ascomycetes community structure and stabili-ty,soil samples were taken from the bare land,the land growing with strong salt-tolerant vegetation(Suaeda salsa,Aeluropus littoralis),and the land growing with light salt-tolerant vegetation(Imperata cylindrica,Arte-misia capillaris)in the Yellow River Delta,and then the physicochemical property detection,high-throughput sequencing and bioinformatics analysis were conducted.It was found that ascomycetes were abundant in the ar-ea,with 10 orders and 67 genera.The diversity and abundance of ascomycetes in soil growing with Artemisia capillaris were the highest,while those in soil growing with Suaeda salsa were the lowest.The ascomycetes community structure was different obviously in the soil growing with different tolerant vegetations.The results of LEfSe analysis showed six(Chaetomiaceae,Chaetomium,Sordariales,Helotiales,Tetracladium,unidentified Helotiales)and two(Emericellopsis,Hypocreales)unique indicator species in light and strong salt-tolerant vegetation growing soil,respectively.Furthermore,the RDA and NMDS analysis showed that the clay particle size,soil nutrients and salt were the main environmental factors influencing the differences in ascomycete com-munity.The genus Torula,which is a member of the phylum Dothideomycetes,was a crucial microorganism for the stability of the whole fungal community in the saline soil environment according to the co-occurrence network analysis.The research results could provide microbial-level scientific supports for species selection and planting pattern optimization in vegetation reconstruction of the Yellow River Delta.