Improving Blast Resistance of Rice CMS Line Taifeng A by Pigm-Based Marker-Assisted Selection Strategy
Deng Jiqi
Huang Jun
Che Fanhao
Zou Yuying
Zhou Geng
Guo Chenglong
Shen Qiang
Li Jiaxin
Liu Jinling
Liu Xionglun
Abstract：To orderly improve the resistance of rice CMS line Taifeng A to rice blast disease,we selected the rice cultivar Gumei 4,known for harboring the broad-spectrum and durable blast resistance gene Pigm,as the donor parent,and employed the maintainer line Taifeng B and its sterile line Taifeng A as the recipient parents,to conduct molecular marker-assisted selection utilizing the selected codominant marker S29742 loca-ted within the Pigm gene.In the Gumei 4 genome,the marker S29742 yielded a 554-bp amplification product,contrasting with a 745-bp product observed in the genomes of Taifeng A and Taifeng B,indicating a pro-nounced polymorphism.By selecting with marker S29742 and integrating field and laboratory identification of resistance to rice blast seedling blight,we successfully screened out two improved sterile lines,Taifeng A-Pigm-1 and Taifeng A-Pigm-2,along with their respective maintainer lines,Taifeng B-Pigm-1 and Taifeng B-Pigm-2.Through evaluations of fertility,agronomic traits and grain quality,we ultimately identified the refined sterile line Taifeng A-Pigm-1,characterized by high resistance to blast disease,significant sterility,stable ste-rility,favorable outcrossing habits and superior agronomic attributes,accompanied by its compatible maintai-ner line Taifeng B-Pigm-1.The results established a foundation for the continued development of new hybrid rice combinations with broad-spectrum,high-level resistance to rice blast disease.
Keywords：Taifeng ACytoplasmic male sterile(CMS)lineRice blast resistancePigm geneMarker assisted selection(MAS)
Publication Date：2025-10-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 28-35 )