Improved QuEChERS/UPLC-MS/MS Method for Detecting Residues of Seven Heterocyclic Amide Fungicides and One Metabolin in Ginseng and Similar Herbal Medicines
Liu Yiping
Liang Jingyun
Ma Shouhong
Abstract：An analytical method was developed for the determination of fluxapyroxad,fluopyram,fluopi-momide,furametpyr,penthiopyrad,thifluzamide,furpylamin fluopicolide and the metabolin 2,6-dichloro-benzamide residues simultaneously in Ginseng(Ginseng Radix Et Rhizoma),Salviae Miltiorrhizae Radix Et Rhizoma American ginseng(Panacis Quinquefolii Radix),Codonopsis Radix through improving pre-treatment process and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)meth-ods.Samples were extracted with acetonitrile.Salviae Miltilorrhizae Radix Et Rhizoma extracting solution per milliliter was cleaned up with 180 mg MgSO4,30 mg PSA(ethylenediamine-N-propylsilane)and 3 mg GCB(SpeedTestTM activited carbon),while the extracting solution of Ginseng,American ginseng and Codonopsis Radix were cleaned up with 20 mg PSA.The purified extracts were detected by UPLC-MS/MS with Waters ACQUITY UPLC® HSS T3 column in positive ionization mode with multiple reaction monitoring and scanning,and the methanol solution containing 0.1％formic acid and aqueous solution containing 0.1％formic acid were used as mobile phase B and A respectively for gradient elute.The results showed that the 8 target compounds showed good linearity in the range of 0.000 1 to 0.1 mg/L with R2 over 0.999.The limits of detection and lim-its quantitation were in the range of 0.000 01～0.000 6 mg/kg and 0.000 2～0.001 0 mg/kg,respectively.The recovery rate was 76％to 120％at three supplemental levels of 0.01,0.10 and 1.00 mg/kg with the relative standard coefficient as 0.5％to 15.7％.This method had the characteristics of fast and simple pre-treatment process,good sensitivity and high accuracy,so it could be used to detect the residue of the seven heterocyclic amide fungicides and one metabolin in Ginseng and three similar herbal medicines.
Keywords：QuEChERSUPLC-MS/MSGinseng and three similar herbal medicinesHeterocyclic am-ide fungicidesPesticide residue
Publication Date：2025-08-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：10( 139-148 )