Effects of Different Phosphorus Levels on Growth,Physiological and Biochemiscal Characteristics and Root Exudates of Impatiens uliginosa
Yan Shun
Zeng Hong
Chen Qian
Huang Linxi
Zhang Xianyan
Yang Yun
Wang Qiong
Abstract：To investigate the effects of different phosphorus nutrient levels on physiology,biochemistry and root exudates of Impatiens uliginosa,and provide theoretical basis for the use of I.uliginosa in artificial wetland wa-ter purification,five phosphorus concentrations[0.05 mmol/L(P0.05),0.10 mmol/L(P0.10),0.20 mmol/L(P0.20),0.25 mmol/L(P0.25)and 1.00 mmol/L(P1.00)]were set in this experiment to analyze their effects on plant height,root-shoot ratio,total phosphorus and chlorophyll contents,root vitality and acid phosphatase(APA)activity of I.uliginosa.The root exudates of I.uliginosa were collected by hydroponics,and the pH value and conductivity of the collected solution were determined,and then the composition of ethyl acetate extract of the root exudates was i-dentified by gas chromatography-mass spectrometry(GC-MS).The results showed that the root-shoot ratio,root vi-tality and APA activity of I.uliginosa showed the significant decline trend with the increase of phosphorus concen-tration,and the maximum values were 1.68,8.54 μg/(g·h)and 35.87 μg/(g·min)respectively,when the phos-phorus concentration was 0.05 mmol/L.The plant height,total phosphorus and chlorophyll contents of I.uliginosa showed the significant increase trend with the increase of phosphorus concentration,and the maximum values were 29.53 cm,4.56 mg/g and 6.34 mg/g respectively,when the phosphorus concentration was 1.00 mmol/L.The main components of I.uliginosa root exudates under different phosphorus concentration treatments included esters,aro-matic hydrocarbons,olefins,quinones and other compounds,and the differences between the components of the root exudates of I.uliginosa under the treatments of P0.25,P0.20 and P0.05 were relatively smaller,which were signifi-cantly different from that under P1.00 and P0.10 treatments.Diisobutyl phthalate(DIBP)was a significantly different substance among different treatment groups,when phosphorus concentration was 0.20 mmol/L,the relative content of DIBP reached the highest of 90.92％.The results of correlation analysis showed that the relative content of DIBP was significantly positively correlated with the root-shoot ratio of I.uliginosa,while it was highly negatively correla-ted with the total phosphorus content,and significantly negatively correlated with phosphorus supply concentration and plant height.It indicated that different phosphorus supply concentration altered the DIBP content of I.uligi-nosa root exudates,which in turn affected the growth and phosphorus absorption of I.uliginosa.
Keywords：Impatiens uliginosaPhosphorus levelRoot exudatePhysiological and biochemiscal char-acteristicsDiisobutyl phthalate(DIBP)
Publication Date：2025-08-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：10( 107-116 )