Microbial Diversity Comparison between Rhizosphere and Non-Rhizosphere Soil from Pyrus betulaefolia in Saline-alkali Land

Mei Yuling; Mou Xiulu; Gong Jiale; Zhu Chenghong; Li Dingli; Zhou Xiaozhi; Yang Yingjie

doi:10.14083/j.issn.1001-4942.2025.08.012

Abstract：Analysis of plant rhizosphere microbial community structure changes under saline-alkali envi-ronment can provide the theoretical basis and practical guidance for finding plant growth promotion rhizosphere microorganisms(PGPRs)which can alleviate saline-alkali injuries effectively.Pyrus betulaefolia is a common rootstock in northern China with strong tolerance to salt and alkali stress.In this study,the rhizosphere soil of P.betulaefolia grown in saline-alkali environment for many years was taken as material,the microbial commu-nity diversity and function were analyzed and forecasted through metagenome technology.The results showed that there were significant differences in the structure and diversity of microbial communities between rhizo-sphere and non-rhizosphere soils,and the species richness and diversities in rhizosphere soil were significantly higher than those in non-rhizosphere soil.The dominant bacteria phyla in the rhizosphere soil were Actinobac-teriota,Proteobacteria,Acidobacteriota and Chloroflexota.The rhizosphere soil was also enriched with more marker microorganisms,including Proteobacteria,Actinobacteriota and Bacteroidetes,as well as many related classes,orders,families and genera belonging to the three phyla.The results of functional annotations showed that the microorganisms enriched in rhizosphere soil had stronger activities of vitamins,amino acids and poly-saccharides metablism,and abilities of stronger cytokinin synthesis and chemical component degradation.The enrichment of these functions might be helpful to enhance the tolerance of plants to saline-alkali and other harsh environments.The study results could lay a foundation for high-quality and efficient cultivation of P.bet-ulaefolia and saline-alkali soil improvement and utilization.