Effects of Arginine on Morphological and Physiological Characteristics of Rice Seedlings under Low Temperature Stress
Zhou Lu
Xiong Jiaming
Liu Ling
Zeng Hongli
Qu Cheng
Pan Sujun
Abstract：This experiment used Oryza sativa'Xiangzaoxian 45'as test material and conducted under the artificially simulated low-temperature(4 ℃)environment.With spraying distilled water followed by nor-mal-temperature(25 ℃)treatment(CK1)and low-temperature(4 ℃)treatment(CK2)as control groups,spraying arginine solutions at different concentrations of 0.025,0.25,2.5 and 25 mmol/L(labeled as T1,T2,T3 and T4,respectively)were set to study their effects on the morphological and physiological character-istics of rice seedlings under low-temperature stress at 12,24 and 36 hours.The results demonstrated that low-temperature stress significantly inhibited plant height,aboveground fresh weight and chlorophyll biosynthesis in leaves.However,appropriate arginine concentrations effectively alleviated these inhibitions.After 12 hours low-temperature treatment,T2(0.25 mmol/L arginine)significantly increased plant height by 6.73％com-pared to CK2,and also increased the total root length,root surface area,root tip number and root branching number by 2.84％,10.06％,3.30％and 4.20％,respectively.After 24 hours low-temperature stress,T2 sig-nificantly enhanced plant height and stem diameter by 15.85％and 6.73％,respectively.After 36 hours of low-temperature treatment,T2 increased the aboveground fresh weight by 10.16％compared with CK2.After 12 hours and 24 hours low-temperature exposure,T2 significantly increased the chlorophyll b and total chlorophyll levels by 59.13％,31.74％and 94.43％,29.95％,respectively,compared to CK2.Arginine application en-hanced the peroxidase(POD)and catalase(CAT)activities in both leaves and roots under low-temperature stress.After 24 hours treatment,the activities of SOD and POD in leaves treated with T2 were significantly higher than those of CK2,with the increase rates as 29.69％and 30.53％,respectively.In sum,spraying 0.25 mmol/L arginine effectively alleviated the adverse effects of low-temperature stress on rice seedling morphologi-cal development and physiological activity,thus enhanced the cold resistance during seedling stage.
Keywords：Rice seedlingArginineLow-temperature stressMorphological characteristicsPhysiologi-cal characteristics
Publication Date：2025-08-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：9( 66-74 )