Effects of Sheep Manure and Diatomite on Physiological Characteristics of Tobacco with Different Genotypes under Lead Stress
Wen Xiaoyang
Lyu Meng
Qin Dehua
Wang Hui
Jin Jianghua
Li Yu'e
Yang Ping
Wen Xuanlin
Liu Ling
Chen Mingcan
Abstract：In this experiment,lead low-enriched tobacco variety Yunyan 87 and high-enriched tobacco varie-ty K326 were used as materials,and five treatments were set as CK(control group),T1(lead 500 mg·kg-1),T2(lead 500 mg·kg-1+sheep manure 1.33 g·kg-1),T3(lead 500 mg·kg-1+diatomite 30 g·kg-1),T4(lead 500 mg·kg-1+sheep manure 1.33 g·kg-1+diatomite 30 g·kg-1).Through pot experiments,the effects of sheep manure,diatomite and their combining application on physiological characteristics of tobacco leaves under lead stress were investigated,including fluorescence characteristics of Fv/Fm(maximum photo-chemical efficiency),chlorophyll content,photosynthetic parameters of Pn(net photosynthetic rate),Tr(transpiration rate)and Gs(stomatal conductivity),antioxidant enzyme activity and related substances con-tents of SOD(superoxide dismutase),MDA(malondialdehyde)and H2O2(hydrogen peroxide),osmotic ad-justment substances contents of proline(Pro)and soluble protein(SP),and root activity.The results showed that,T1 treatment,namely lead stress,could inhibit the physiological activity of tobacco.On the whole,T4 treatment could significantly increase the chlorophyll content,Fv/Fm,Gs,Tr and Pn of Yunyan 87 leaves,significantly increase the Fv/Fm and Tr of K326 leaves.That was,sheep manure+diatomite treatment could significantly promote the photosynthesis of tobacco,and reduce the MDA,Pro,H2O2 and soluble protein con-tent and SOD activity of Yunyan 87 leaves under lead stress at the same time,thus reducing the toxicity of lead stress to tobacco.The treatments of sheep manure,diatomite and sheep manure+diatomite all could improve the vitality of tobacco root system under lead stress.In conclusion,applying appropriate amount of sheep ma-nure+diatomite in the soil could improve the resistance of different genotypes of tobacco to lead stress,and contribute to the growth,development and physiological activity of tobacco.
Keywords：TobaccoLead stressSheep manureDiatomitePhotosynthetic characteristicsPhysiolog-ical characteristics
Publication Date：2025-07-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：10( 123-132 )