Effects of Aluminum Stress on Seed Germination and Seedling Growth and Physiological Characteristics of Three Legume Forage Grasses
Liu Danyang
Zhao Lili
Long Sisi
Ou Jing
Abstract：In order to understand the growth and physiological responses of three legume forage grasses(Trifolium repens L.,Medicago sativa L.,Lotus corniculatus L.)to aluminum stress,and to compare their differences in aluminum tolerance,six aluminum concentrations of 100,200,300,500,700 and 1 000 mg·L-1 were set in this study.The seed germination,seedling growth and physiological indexes were measured,the e-valuation indexes of aluminum tolerance were screened out through principal component analysis,and the alu-minum tolerance was evaluated by membership function method.The results showed that low concentration alu-minum stress(100 or 200 mg·L-1)had a certain promoting effect on seedling growth and biomass of T.repens M.sativa and L.corniculatus.As the increase of aluminum concentration,the seed germination and growth in-dicators of the three legume forage grasses significantly decreased,showing the greatest inhibition to root sys-tem.Moreover,as the increase of aluminum concentration,the contents of soluble sugar and proline as well as the activities of peroxidase(POD)and catalase(CAT)in the three legume forages showed the trend of increas-ing first and then decreasing,reaching their maximum values at aluminum concentration of 200 or 300 mg·L-1.The relative root length,relative germination index,relative viability index,relative malondialdehyde content and relative peroxidase activity selected by principal component analysis could be used as evaluation indicators for aluminum tolerance of T.repens,M.sativa and L.corniculatus.The average membership function value of the five indicators calculated by membership function method could be used to identify the aluminum tolerant capacity,and the results showed that T.repens had the strongest aluminum tolerance,followed by M.sativa,and the aluminum tolerance of L.corniculatus was the worst.These results could provide the theoretical basis and certain reference for screening aluminum-tolerant leguminous forage germplasms and breeding new varie-ties in the southwest region of China.
Keywords：Legume forage grassAluminum stressSeed germinationOsmoregulation substancesAn-tioxidant enzymes
Publication Date：2025-07-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：9( 83-91 )