Effects of Black Shank Infection on Transcriptome and Metabolome of Sun-Cured Tobacco in Daning
Qi Shiqing
Shou Anfa
Tian Mengqiang
Shi Baofeng
Yang Mengquan
Liu Guoshun
Yin Quanyu
Yu Jinheng
Abstract：Daning sun-cured tobacco is a species of good-quality yellow sun-cured tobacco in Hezhou,Guangxi,and it is the excellent raw material for cigarette production.However,it has extremely low resistance to black shank disease.This study investigated the response mechanism of Daning sun-cured tobacco to black shank disease by comparing the differentially expressed genes and metabolites between healthy and black shank-infected leaves through transcriptomic and metabolomic analyses.The results showed that a total of 7 194 significantly differentially expressed genes were identified through transcriptomic analysis,and 198 sig-nificantly different metabolites were detected through metabolomic analysis.The combined transcriptomic and metabolomic analysis revealed that:① Plant hormone signal transduction and phenylpropanoid biosynthesis pathways played important roles in the defense of Daning sun-cured tobacco against black shank infection.②It was speculated that metabolic pathways such as aminoacyl-tRNA biosynthesis,cyanogenic amino acid metabo-lism,valine,leucine and isoleucine degradation,cysteine and methionine metabolism,and arginine and pro-line metabolism were related to the response of Daning sun-cured tobacco to black shank stress.This study elu-cidated the major gene transcriptional regulatory networks and metabolic pathways of Daning sun-cured tobacco in response to black shank infection,which could provide a theoretical basis for research on improving the re-sistance of Daning sun-cured tobacco to black shank disease.
Keywords：Daning sun-cured tobaccoBlack shank diseaseTranscriptomeMetabolomePlant hor-mone signal transductionPhenylpropanoid biosynthesis
Publication Date：2025-06-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：11( 37-46,63 )