Identification of R2R3-MYB Gene Family and Their Expression Analysis under Verticillium Wilt Stress in Gossypium barbadense
Hu Xingui
Zhao Jieyin
Lu Jiaxin
Chen Quanjia
Qu Yanying
Abstract：R2R3-MYB transcription factors play crucial roles in the process of plants responding to stress.In this study,based on the genomic data of Gossypium barbadense,the members of the R2R3-MYB gene family in G.barbadense were systematically identified.Bioinformatics methods were used to analyze the phyletic evolution,structure,physicochemical properties,and cis-acting elements of the promoter of the R2R3-MYB gene family in G.barbadense.Five candidate genes for Verticillium wilt resistance were screened out using tran-scriptome expression profiles,and the expression patterns of each candidate gene under the induction of Verti-cillium wilt,salicylic acid(SA)and methyl jasmonate(MeJA)were analyzed by real-time fluorescence quan-titative PCR.The results showed that there were 98 R2R3-MYB genes in the genome of G.barbadense,which distributed on 25 chromosomes.The phylogenetic tree analysis indicated that the members of the R2R3-MYB gene family in G.barbadense could be divided into three large subgroups,and they were relatively conserved in evolution.Promoter analysis revealed multiple cis-acting elements related to plant hormones and stress respon-ses.The expression pattern analysis after Verticillium wilt stress showed that all the R2R3-MYB genes in G.barbadense had different degrees of differential expression after Verticillium wilt stress.Five candidate genes for Verticillium wilt resistance in G.barbadense(GbMYB-21,GbMYB-34,GbMYB-39,GbMYB-76,and GbMYB-83)were screened out,which were all induced by Verticillium wilt stress,SA and MeJA.In conclusion,this study demonstrated that the R2R3-MYB gene family in G.barbadense played an important role in cotton disease resistance,which could provide a theoretical basis for further research on the functions of R2R3-MYB genes in G.barbadense and the molecular mechanism of cotton resistance to Verticillium wilt.
Keywords：Gossypium barbadenseR2R3-MYB gene familyBioinformatics analysisVerticillium wilt stressExpression analysis
Publication Date：2025-06-30
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：14( 1-14 )