Cloning and Expression Analysis of OsIAA1 and OsIAA24 Genes in Rice Seeds

Wang Na; Jin Zuying; Zhang Lulu; He Xiahong; Wang Shu

doi:10.14083/j.issn.1001-4942.2025.05.003

Abstract：The Aux/IAA(auxin/indole-3-acetic Acid)genes are key genes in plant auxin regulation,which play an essential role in regulating plant growth and development by regulating auxin signaling,espe-cially in promoting seed development.To investigate the functions of Aux/IAA genes in rice seeds under differ-ent cultivation modes(dryland and traditional paddy cultivation),this study successfully cloned two Aux/IAA genes from rice(Dianheyou 615)seeds designated as OsIAA1 and OsIAA24,which had full-length cDNA se-quences of 600 bp and 660 bp,and encoded 199 and 219 amino acids,respectively.The results of bioinforma-tics analysis indicated that both OsIAA1 and OsIAA24 were hydrophilic and unstable proteins with no signal peptides or transmembrane domains,containing four typical conserved domains of the AUX/IAA gene family and both were located in nucleus.The results of system evolution analysis indicated that the amino acid se-quences of OsIAA1 and OsIAA24 exhibited a similarity of 99.50%and 98.20%to those of the Oryza glaberri-ma,respectively.Results of qRT-PCR analysis revealed that the expression levels of OsIAA1 and OsIAA24 were significantly higher under dryland cultivation compared to paddy cultivation,and it was speculated that they might be involved in the regulation of seed development and play a critical role in drought resistance in rice.The findings of this study could provide theoretical supports for further elucidating the molecular mecha-nisms underlying seed development in Dianheyou 615 under dryland and paddy cultivation conditions.