Molecular Detection and Genetic Effect Analysis of TaDIR-B1 Gene Associated with Fusarium Crown Rot in Wheat

Zhao Qi; Guo Can; Cheng Dungong; Li Faji; Li Haosheng

doi:10.14083/j.issn.1001-4942.2025.04.006

Abstract：Fusarium crown rot(FCR)has become one of the most important diseases in the main wheat production region of China.Detecting the distribution of FCR resistance alleles and analyzing their genetic effects could provide guidance and available resources for wheat FCR resistance breeding.TaDIR-B1 gene has been reported to be involved in the regulation of wheat lignin synthesis,whose loss-of-function could signifi-cantly improve lignin synthesis,and then improve the resistance to FCR.In the present study,a total of 106 wheat varieties(lines)originated from home and abroad were used as materials,the disease severity was in-vestigated by inoculation test with FCR in field,and the distribution of different variants and their genetic effects on FCR were analyzed by TaDIR-B1 sequencing.The results showed that among the 106 materials,73 belonged to mutant type(TaDIR-B1b)which accounting for 68.87％of the total,and 33 belonged to wild type(TaDIR-B1a)which accounting for 31.13％of the total.The mean disease severity of FCR in the materi-als carrying TaDIR-B1b was lower than that in the materials carrying TaDIR-B1a,and the differences in three environments and the mean value were significant(P＜0.05)or extremely significant.The proportion of Ta-DIR-B1b materials in Shandong and Hebei Province was relatively higher.Jimai 1703,Mantol,Baimangmai,Shi 4185,Boomer and Azulon carried TaDIR-B1a gene and had lower disease severity of FCR,which could be used as parents for resistant variety breeding.