Cloning and Bioinformatics Analysis of PhMYB14 Gene in Populus hopeiensis

Pang Linlin; Zhang Qi; Dai Jinling; Bai Yu'e

doi:10.14083/j.issn.1001-4942.2025.04.002

Abstract：MYB transcription factors play important roles in plant growth and development in ways of in-teractions,signal transduction,and gene expression regulation.In this study,using the seedlings of Populus hopeiensis as materials,PhMYB14 gene was cloned,its bioinformatic analysis was performed,and its tissue-specific expression in P.hopeiensis was analyzed.The results showed that the length of coding sequence(CDS)of PhMYB14 gene was 723 bp,encoding 240 amino acid residues.The PhMYB14 protein had the mo-lecular weight of 27 512.87 Da and the theoretical isoelectric point of 5.17,and was an unstable hydrophilic protein with no signal peptide and transmembrane structure.Homologous sequence alignment and protein phy-logenetic tree analysis showed that PhMYB14 was the most closely related to Populus trichocarpa and Populus alba,both of which also belonged to the genus Populus,followed by Salix sinopurpurea and Salix suchowensis.Prediction of subcellular localization suggested that PhMYB14 was mainly locatted in chloroplasts.The promot-er region of PhMYB14 contained various cis-acting elements including photoresponsive elements and methyl jasmonate response elements.Real-time fluorescence quantitative analysis(qRT-PCR)showed that PhMYB14 was expressed in the roots,stems and leaves of poplar seedlings with the highest expression level in leaves.The results of this study could provide a reference for further study of the functional mechanism of PhMYB14 in the resistance to diseases and insect pests of P.hopeiensis.