Effects of Hydrogen-Rich Nutrient Solution on Morphology and Physiological Activities of Tomato Seedlings under Salt Stress

Jiang Yuetao; Zhang Shunzhi; Wang Fu; Wang Hui

doi:10.14083/j.issn.1001-4942.2025.02.009

Abstract：In this study,the main morphological and physiological indexes of tomato seedlings under 100 mmol/L NaCl stress(S treatment)were determined by applying different concentrations of H2 in 1/2 Hoag-land nutrient solution.The H2 saturability was set as 25％,50％,75％and 100％,which was marked as 0.25H,0.50H,0.75H and 1.00H.The results showed that compared with S treatment,higher concentrations of hydrogen treatments(S+0.50H,S+0.75H)significantly increased the plant height,stem diameter,aboveground dry weight,underground dry weight and seedling index of tomato seedlings,the photosynthetic pigment content in leaves,and the net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of leaves,and significantly reduced the intercellular CO2 concentration(Ci);they also significantly increased the activities of SOD,POD and CAT,but there were no significant difference with the control(CK,1/2 Hoagland nutrient solution).Moderate concentration of hydrogen treatment(S+0.50H)significantly im-proved the maximum photochemical quantum yield of the reaction center PS Ⅱ(Fv/Fm),actual light quantum yield of PS Ⅱ(ΦPS Ⅱ),coefficient of photochemical quenching(qP)and apparent electron transport rate(ETR)of tomato seedling leaves under salt stress,and significantly reduced the initial fluorescence(Fo).In addition,with the increase of hydrogen concentration in nutrient solution,the content of malonaldehyde in to-mato seedling leaves under salt stress decreased gradually,but all were significantly higher compared to CK.In summary,hydrogen-rich nutrient solution could alleviate the inhibition of salt stress to morphology and physio-logical activities of tomato seedlings,and the S+0.50H treatment had the best effects.