Identification of Tomato HD-Zip Family Genes and Expression Analysis of HD-Zip Ⅲ Subfamily Genes under Abiotic Stresses

Zhang Xin; Guo Xudong; Zang Rina; Zhang Shunzhi; Zhu Wenying; Wang Fu; Wang Hui

doi:10.14083/j.issn.1001-4942.2025.02.002

Abstract：In order to explore the characteristics of tomato HD-Zip family members and the role of HD-Zip Ⅲ subfamily members in response to abiotic stresses,we performed genome-wide identification and ex-pression analysis of tomato HD-Zip family under abiotic stresses.The results showed that a total of 56 HD-Zip family genes were screened out from tomato,which were unevenly distributed on 12 chromosomes.These genes were divided into four subfamilies;Ⅰ,Ⅱ,Ⅲ and Ⅳ,and the genes in the same subfamily had similar physi-cochemical properties,gene structures,conserved motifs and conserved domains.The prediction results of the main cis-acting elements of tomato HD-Zip family genes showed that MeJA responders appeared with the high-est frequency.MeJA could induce the synthesis of defensive compounds and initiate the expression of patho-genesis-related genes related to systemic acquired resistance and local resistance,so these genes might play important roles in tomato stress resistance.Collineearity analysis showed that 30 genes of the tomato HD-Zip family had collinearity,while 34 pairs of genes had collinearity between tomato and Arabidopsis HD-Zip family genes.The results of gene expression analysis showed that the genes of HD-Zip Ⅲ subfamily expressed in the roots of tomato plants,and responded to the abiotic stresses such as cold,salt,drought and ABA.Among which,the expression of Soly03g120910 was significantly increased under cold stress,and the expression level reached the peak at 6 hours of treatment,which was 10.3 times higher compared to 0 hour of treatment.The expression of Soly03g120910 was also up-regulated by drought stress,and the expression level increased by 1.6 times at 6 hours of treatment compared with 0 h.Solyc11g069470 had the strongest response to salt stress,and the expression level was up-regulated by 1 time at 3 hours of treatment compared with 0 h.Soly08g066500 had the strongest response to ABA treatment,and the expression level was up-regulated by 5.4 times after 6 h treatment compared with 0 h.The results of this study could lay a foundation for further study of the mechanism of HD-Zip family genes in plant stress response.