Identification and Evaluation of Salt Tolerance of Radish during Germination

Cheng Yide; Liu Chen; Liu Xianxian; Xu Wenling; Che Yali; Wang Shufen

doi:10.14083/j.issn.1001-4942.2025.01.004

Abstract：In order to evaluate and identify the salt tolerance of radish in early stage,this study used 6 different radish germplasms as materials and screened the appropriate salt concentration to evaluate the salt tol-erance at germination stage.Based on the screened salt concentration,the stress tests were carried out on 107 radish germplasms to analyze its effects on 8 characters including germination potential,germination rate,rela-tive germination potential,fresh weight of sprout,root length of sprout,relative root length,sprout length and relative sprout length.Then the salt tolerance at germination stage was comprehensively evaluated by correla-tion analysis,principal component analysis,membership function method and cluster analysis.The results showed that when NaCl concentration was 200 mmol/L,the germination potential and germination rate of all the six radish germplasms decreased significantly,but the germination rate of most materials was still about 60％,so it was chosen as the appropriate concentration for salt tolerance evaluation.At this salt concentration,107 radish germplasms at germination stage were treated and all the 8 characters showed some variation,a-mong which,the coefficient of variation of germination potential was the largest and that of sprout length was the smallest.Under salt stress,significantly positive correlations were observed among germination potential,germination rate,root length of sprout,relative germination rate,relative root length and relative sprout length.The principal component analysis results showed that the cumulative contribution rate of the first three principal components was as high as 80.687％.The membership function method and cluster analysis were used to rank and cluster the tested radish germplasms respectively,and their results were in good consistency.Five germplasms with strong salt tolerance were selected,which were No.101(Chunbaiyu),No.70(Yang-zhou Yuanbai),No.89(Degaoliangfenghong),No.79(Huaxiayipin)and No.91(Yidianhong).The results of this study could provide data support for salt tolerance evaluation and new insight for new salt-tolerant varie-ty breeding of radish in the future.