Transcriptome Analysis of Blue-Purple Rosa hybrid Cultivar'Nightingale'in Flowering Period
Sun Junfei
Meng Xianshui
Zhang Jingying
Ren Fengshan
Li Yi
Abstract：The expression patterns of genes related to flower color were explored at the molecular level by using petals at 5 different developmental stages of Rosa'Nightingale'as materials based on transcriptome a-nalysis.The testing results indicated that 386,1 492,830 and 255 upregulated differentially expressed genes(DEGs)and 607,1 849,150 and 783 downregulated DEGs were found through the comparison of initial blooming stage with bud stage(N_S2 vs N_S1),half blooming stage with initial blooming stage(N_S3 vs N_S2),full blooming stage with half blooming stage(N_S4 vs N_S3),flower falling stage with full blooming stage(N_S5 vs N_S4),respectively.A total of 15 DEGs were found in the flavonoids and anthocyanin biosyn-thesis pathway,and the biosynthesis of flavonoids and anthocyanin reached the peak during the half blooming to full blooming period.The expression trend of R2R3MYB transcription factors MYB6 and MYB113 were sim-ilar to those of flavonoids and anthocyanin biosynthesis related genes,which might be involved in the regula-tion of anthocyanin synthesis.In the carotenoid biosynthesis pathway,a total of 14 DEGs were found,and the biosynthesis of carotenoids reached the peak during the initial blooming to half blooming period.It was found that the carotenoids biosynthesis occurred earlier than that of anthocyanin,which could avoid the interference of yellow on the blue-purple flower color of Rosa'Nightingale'.
Keywords：Rosa'Nightingale'Blue-purple flower colorTranscriptome analysisFlavonoidAntho-cyaninCarotenoids
Publication Date：2024-11-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 13-20 )