Bioinformatics and Expression Analyses of VvLBD35 and VvLBD36 Genes in Grape
Li Ying
Li Gaoqing
Gao Minggang
Xu Ruirui
Abstract：The LATERAL ORGAN BOUNDARIES DOMAIN(LBD)family is a plant specific transcript factor family,which contains highly conserved LOB domain and involves in growth,metabolism and a variety of stress responses in the plant.In this study,the gene structure and evolution relationship of VvLBD35 and Vv-LBD36 in grape LBD gene family were systematically analyzed by bioinformatics method,and their tissue ex-pression patterns and induced expression patterns under drought,high salt,high temperature and low tempera-ture stresses were detected by qRT-PCR.The results showed that both VvLBD35 and VvLBD36 contained α-helix,random curl and β-corner structures,and VvLBD36 contained more β-fold structure.VvLBD35 and Vv-LBD36 had higher homology with the LBD proteins of Arabidopsis,rice,maize,citrus,tomato and so on.The promoter region of VvLBD35 and VvLBD36 contained a variety of response elements related to stress,hormone and growth and development.Both VvLBD35 and VvLBD36 were expressed in different degrees in roots,stems and leaves,and both had the highest expression level in leaves,which was about 2～8 times of that in roots and stems.Analysis of induced expression pattern showed that VvLBD35 was the most significantly induced by high temperature which was down-regulated to about 6％of that in the control,while the expression of Vv-LBD36 was the most significantly induced by high salt and high temperature,which was up-regulated to 5.67 and 14.29 times of that in the control,respectively.These results provided the experimental foundation for fur-ther revealing biological functions of VvLBD35 and VvLBD36 in grape.
Keywords：GrapeLATERAL ORGAN BOUNDARIES DOMAIN(LBD)familySequence analysisExpression analysisAbiotic stress
Publication Date：2024-08-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 23-29 )