Correlation Analysis of Tumorigenesis and Heat Shock Protein Expression in Lung of Chickens Infected by Marek's Disease Virus
Li Yanlan
Zhu Xihui
Wang Zhiwei
Pang Zheyu
Li Yubao
Abstract：In order to investigate the relationship between Marek's disease virus(MDV)and tumorigene-sis and development,a chicken Marek's disease(MD)model was established in this study,and the localiza-tion,transcription and expression levels of the tumor-associated protein heat shock protein 60(HSP60)in chicken lung tissues were determined by using histo pathology,immunohistochemistry,fluorescence quantita-tive PCR and enzyme-linked immunosorbent assay(ELISA)methods.The results showed that HSP60 was strongly expressed in the cytoplasm of chicken lung tumor cells in the takedown group.The HSP60 mRNA transcript level in the takedown group was extremely significantly higher than that in the control group during the test cycle(P＜0.01),and showed an overall parabolic pattern with a downward opening,namely,contin-ued to increase during 7 to 28 days of age,and reached the maximum at the 28 days of age,which was 1.39 and 1.44 folds of that of the blank and vaccine control groups,respectively,after that,it decreased.The ex-pression of HSP60 was extremely significantly higher in the takedown group than that in the blank control group from 7 to 28 days of age(P＜0.01),and the difference between them was not significant after 35 days of age.These results could lay a foundation for further research on the biological role of HSP60 in tumorigenesis.
Keywords：Marek's diseaseHeat shock proteinHistopathologyImmunohistochemistry
Publication Date：2023-12-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 150-155 )