Analysis of Rhizosphere Soil Properties and Fungal Community Characteristics of Greenhouse Strawberry in Different Continuous Cropping Years

Tian Chunli; Yuan Bingqiang; Yao Lijuan; Xiao Yanyun

doi:10.14083/j.issn.1001-4942.2023.12.014

Abstract：Soil fungi play important roles in soil ecological environment.In agricultural production,long-term monoculture and continuous cropping can lead to changes in fungal community structure.However,the effects of long-term continuous cropping on strawberry plant health and rhizosphere fungal community composi-tion are still unclear.In this study,the Illumina-MiSeq technique was used,and the rhizosphere soil properties and fungal community structure characteristics of greenhouse strawberry between non-continuous cropping(CK)and continuous cropping with different years(1,2,4,7,9,11 a)were analyzed.The results showed that the soil properties changed significantly after continuous cropping of strawberry,and the soil nutrient con-tent began to decrease significantly after 4-year continuous cropping.High-throughput sequencing results showed that the richness and diversity of soil fungal communities increased with the increase of continuous cropping years.Specifically,the abundance of Fusarium,Humicola and Guehomyces increased significantly af-ter strawberry continuous cropping,while that of Funneliformis and Arthrobotrys decreased from the 4th year of continuous cropping.Spearman correlation analysis showed that soil pH,available potassium and available ni-trogen were the main factors to determine the structure of beneficial bacteria and pathogenic bacteria in straw-berry continuous cropping system.Furthermore,the redundancy analysis(RDA)results showed that soil pH and available nitrogen were the main factors determining the abundance of Funneliformis and Arthrobotrys in continuous cropping system,and available potassium was the main soil factor affecting the abundance of Gue-homyces,Humicola and Fusarium.The results of this study could provide theoretical references for sustainable development of strawberry planting and continuous cropping soil management.