Effect of Host Conversion on Diversity of Endosymbiotic Bacteria in Bemisia tabaci

Gao Huanhuan; Zhao Na; Gao Zhongqiang; Zhou Xianhong; Zhuang Qianying; Zhang Ansheng; Zheng Chengmin

doi:10.14083/j.issn.1001-4942.2023.12.012

Abstract：In order to make clear the differences in endosymbiotic bacteria diversity of Bemisia tabaci in different host plants,two B.tabaci populations fed with tomato and cotton were established in this experiment.The diversity of endosymbiotic bacteria in the adults of the two B.tabaci populations and the infection condi-tion of dominant symbiotic bacteria were determined by PacBio sequencing platform.The results showed that the dominant symbiotic bacteria in B.tabaci population fed with tomato were Portiera,Rickettsia and Hamil-tonella.The species of symbiotic bacteria and the number of amplicon sequence variants(ASVs)decreased obviously when B.tabaci were transferred to cotton from tomato,and the dominant symbiotic bacteria increased to four genus as Portiera,Rickettsia,Hamiltonella and Cardinium with the relative abundance changing signifi-cantly.The highest infection rate of Protiera was the highest in the two B.tabaci populations,followed by Rick-ettsia,Hamiltonella and Cardinium,andthe co-infectionof three or four species of symbiotic bacteria was the main form.The co-infection richness of symbiotic bacteria increased significantly when B.tabaci were trans-ferred to cotton from tomato.In conclusion,host conversion had significant effect on the diversity of endo sym-biotic bacteria in B.tabaci.These results could provide theoretical bases for researching the co-evolutionary re-lationship between B.tabaci,endosymbiotic bacteria and host plants.