Genetic Diversity Analysis and DNA Fingerprint Construction of Pomegranate Varieties Based on SSR Molecular Markers
Feng Lijuan
Cheng Yun
Wang Chuanzeng
Jiao Qiqing
Yin Yanlei
Abstract：In order to identify the genetic diversity of major pomegranate varieties and construct their DNA fingerprints,30 pomegranate varieties were used as materials,the agarose and capillary electrophoresis were used to screen polymorphic primers,and the SSR molecular marker identification system were construc-ted.Then the DNA fingerprints of the pomegranate varieties were constructed by using the tandem codes con-taining sample,primer and allele information.The results showed that ten pairs of primers with clear and sta-ble bands and high polymorphism were selected from 36 pairs of primers.Using them,48 alleles were detected with the mean of 4.8 per every pair of primer.The average observed heterozygosity and expected heterozygosity were 0.50 and 0.60,respectively.The Shannon's index and Nei's diversity index were 1.11 and 0.59,re-spectively.The polymorphism information content ranged from 0.33 to 0.72,with the mean of 0.53.The cluster analysis results showed that the 30 varieties could be classified into 3 groups,among which,the group A in-cluded 16 varieties,the group B included 8 varieties,and the group C included 6 varieties.Based on the in-formation of samples,primers and alleles,the specific fingerprint codes and their two-dimensional codes of the 30 varieties were constructed.The results of this study could provide theoretical bases for molecular identifica-tion,development and utilization of germplasm resources of pomegranate.
Keywords：Pomegranate(Punica granatum)varietySSR molecular markerGenetic diversityDNA fingerprint
Publication Date：2023-12-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 26-33 )