Identification and Expression Analysis of PAO Gene during Fruit Development and Storage of Ziziphus jujuba
Li Lili
Fan Dingyu
Yang Lei
Jin Juan
Hao Qing
Abstract：Polyamine oxidase(PAO)is a flavin adenine dinucleotide dependent enzyme that catalyzes the catabolism of polyamines(PA)and plays a crucial role in plant growth,development and stress response.The genome-wide screening of PAO genes in jujube was conducted,and three ZjPAO genes(ZjPAO1,ZjPAO2,ZjPAO3)were identified,then their conserved domains,gene structures,chromosomal positions,evolutionary relationships,subcellular localization and promoter cis acting elements were analyzed.The results showed that the three ZjPAO genes were distributed on Chr4,Chr5 and Chr8,respectively,with relatively conservative structural characteristics and the Amino-oxidase domain.By constructing the phylogenetic trees of the PAO genes of Arabidopsis,tomato,peach and jujube,we found that they could be divided into four catego-ries,and the ZjPAO geneswere closely related to the PAO genes in peach.The promoter of the ZjPAO genes contained various cis acting elements related with such as hormones and stress responses,which were predicted to play roles in jujube growth and development.In addition,the transcriptome data analysis showed that both ZjPAO1 and ZjPAO2 expressed at different stages of jujube fruit development.Under exogenous salicylic acid treatment,the ZjPAO1 gene was significantly induced to express.The results of this study could contribute to understanding the mechanisms of delaying ripening and aging of fresh jujube fruits.
Keywords：Ziziphus jujubaPolyamine oxidase(PAO)Gene identificationBioinformatics analysisGene expressionFruit growth and developmentPost-harvest storage
Publication Date：2023-12-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 9-16 )