Bioinformatics and Functional Analysis of ZmWRKY36 Gene in Maize
Wang Wenli
Mu Chunhua
Zhang Fajun
Liu Xia
Leng Bingying
Yao Guoqi
Yan Zhenwei
Liu Wei
Wang Yun
Zhang Enying
Abstract：As one of the largest families of transcription factors in the plant,the WRKY proteins play crucial roles in plant growth and development,defense regulation and stress response.In this research,we cloned a maize WRKY transcription factor gene ZmWRKY36,and conducted the bioinformatics and functional analysis.The results showed that the open reading frame of ZmWRKY36 gene was 1 002 bp,and it encoded 333 amino acids.The molecular weight of ZmWRKY36 was 35.8 kDa,and its isoelectric point was 5.66,so it belonged to an acidic protein;its instability coefficient was 67.16,indicating that it was a highly unstable pro-tein;its hydrophilicity index was-0.526,indicating that it was a hydrophilic protein;it contained one WRKY domain and one C2 HC zinc-finger motif,so it was a member of the subgroupⅢfamily of WRKY transcription factor;it showed close relationships with SbWRKY64,PmWRKY4 and SvWRKY4.Further analysis results showed that ZmWRKY36 protein was localized in the nucleus and had strong transcriptional activation activity.The results of qRT-PCR indicated that the expression of ZmWRKY36 gene was tissue-specific with higher level in root and leaves,and was significantly induced by drought stress and salt stress,which suggested that the transcription factor ZmWRKY36 might play a key role in the process of maize resisting environmental drought and salt stresses.
Keywords：MaizeZmWRKY36Bioinformatics analysisFunctional analysisDrought stressSalt stress
Publication Date：2023-12-28
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：8( 1-8 )