Cloning and Sequence Analysis of FAE1 Gene in Cultivated Peanut
Zhang Xin
Sun Quanxi
Wu Qi
Wang Xiuzhen
Hu Dongqing
Tang Yueyi
Wang Zhiwei
Song Guosheng
Xu Jianzhi
Yin Dongmei
Wang Chuantang
Abstract：Fatty acid elongation 1 (FAE1)is a key enzyme that controls the elongation of fatty acids and plays a crucial role in the biosynthesis of erucic acid.In this study,two FAE1 genes,FAE1 -A and FAE1 -B,were cloned from two genomes of the cultivated peanut,respectively.FAE1 had only one open reading frame,no introns,and the complete coding sequence of the gene is 1 536 bp,encoding 511 amino acids.The bioinformatics analysis results showed that the secondary structure of FAE1 protein was mainly α-helix;there was no signal peptide,indicating that FAE1 was not secretory protein,and it worked in cells and mainly exis-ted in endoplasmic reticulum;the instability coefficient of FAE1 was lower than 40 and the total mean hydro-philic coefficient was negative,so FAE1 was unstable hydrophilic protein.The study may facilitate peanut breeding for low erucic acid content.
Keywords：FAE1Erucic acidPeanutGene cloningBioinformatics analysis
Publication Date：2018-06-02
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 35-40 )