Construction of Rapid Propagation TechnologySystem for Trichosanthes kirilowii Maxim
Ruan Jian
Shan Lei
Fan Zhongxue
Yang Lianqun
Jiao Qiqing
Peng Zhenying
Abstract:In order to overcome the technical limitation of tissue culture and rapid propagation and real -ize the large -scale production of high -quality seedlings of Trichosanthes kirilowii Maxim, the first generation of virus -free Trichosanthes kirilowii seedlings of the variety Yuelou 2 was used as the material.After inducing axillary buds to seedlings, we induced root with seedlings directly , skipping the traditional subculture process . The results showed that the emergence rate of axillary buds was as high as 99% under the concentration of 6 -BA as 0.5 mg/L and IAA as 0.2 mg/L.When the concentration of IBA was 0.5 mg/L, the rooting rate was 88%, and the average number of lateral roots was the highest .The survival rate of refined seedlings could reach 90%.In conclusion, the mass production of Trichosanthes kirilowii Maxim seedlings could be realized by using this rapid progation technology system .
Keywords:Trichosanthes kirilowii MaximTissue cultureRapid propagationHormone combinationAxillary bud induction
Publication Date:2018-05-02
Online Publishing Date:2026-05-22(First online date of this platform, not the publication date of the document)
Pages:5( 46-50 )
Shandong Agricultural Sciences

Shandong Agricultural Sciences

ISTIC
ISSN:1001-4942
Year, Vol.(Issue):2018,50(5)