Effects of Seed Soaking with Lanthanum and Watering Sodium Selenite Solution on Antioxidase Activities and Soluble Protein Content in Wheat Seedlings under Copper Stress
Shi Zhipeng
Zhang Xiusheng
Shi Huaichao
Zhou Xiaoyan
Abstract：The effects of seed soaking with lanthanum nitrate and watering sodium selenite on the SOD and POD activities and soluble protein content in Jimai 22 and Shannong 28 seedling leaves under copper stress were investigated using the sandculture method. The results showed that 100 mg /L copper sulfate treatment reduced the activities of SOD and POD and increased the content of soluble protein in wheat leaves, which poisoned wheat seedlings. For relieving the inhibition to antioxidase activity in Jimai 22 and Shannong 28 under copper stress, the optimum soaking concentration of lanthanum nitrate were 30 ～ 50 mg /L and 30 ～100 mg /L, and the optimum watering concentration of sodium selenite were 15 ～ 25 mg /L and 5 ～ 25 mg /L, and the optimum compound treatment conditions were 100 mg /L lanthanum with 5 ～ 25 mg /L sodium selenite and 50 mg /L lanthanum with 5 ～ 25 mg /L sodium selenite, respectively. The sensitivity of the two wheat variety to lanthanum soaking and watering sodium selenite solution was different. Shannong 28 had better antioxidase system to resist high concentration copper stress. The single and compound effects of lanthanum seed soaking and watering sodium selenite on the SOD and POD activities of the two wheat variety seedlings under the high concentration of copper stress showed promoting at low concentration and inhibiting at high concentration. Lanthanum soaking treatment improved the tolerance of wheat seedlings to sodium selenite, and their compound effects were complementary on enhancing the SOD and POD activities. These results indicated that lanthanum and selenium might increase the activity by not promoting the expression of SOD synthase genes, but by conformation changing or chemical modification, and the effects of dose followed the Hormesis effect. We concluded that soaking seeds with lanthanum and watering sodium selenite solution at optimum concentration could effectively relieve the oxidative damage to wheat seedlings under copper stress.
Keywords：WheatLanthanumSeleniumCopper stressAntioxidaseSoluble protein
Publication Date：2018-04-02
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：9( 64-72 )