Construction of Mutant Library of Bacillus amyloliquefaciens Bs-18 with the Transposon TnYLB-1
Sun Shuqin
Sun Bingbing
Yang Xiurong
Zhang Wei
Huo Jianfei
Abstract：Bacillus amyloliquefaciens Bs-18 is a biocontrol strains against cucumber powdery mildew with significant control effect. In this paper, the mutant library of Bs-18 was constructed with the shuttle plasmid pMarA carrying transposon TnYLB-1 by the electric shock method to deeply study the molecular mechanism of the biocontrol strain. Through screening transpositional temperature and time, the maximum transpositional success rate was obtained after induced at 50℃ for 16 hours. The mutants were verified by PCR. The construction of Bs-18 mutant library laid a foundation for further study on its biocontrol function genes and mechanism.
Keywords：Mutant libraryTransposonBiocontrol function genes
Publication Date：2018-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )